Thermo Fisher Scientific MCP-4 Monoclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT

Preparation:
Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13).
Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography.

Sandwich ELISA:
Assuming 100 µL/well, a concentration of 2.0–4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5–1.0 µg/mL.

Western Blot:
To detect Human MCP-4 (CCL13) by Western Blot analysis, this antibody can be used at a concentration of 0.25–0.50 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Human MCP-4 (CCL13) is 0.5–1.0 ng/lane, under reducing or non-reducing conditions.

Target Information

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFα. MCP-4 is a ligand for three different G protein–coupled receptors (CCR2, CCR3, and CCR5). MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils, and basophils, and this signaling is associated with allergic responses.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.