Thermo Fisher Scientific MCP-4 Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.50 µg/mL

-

ELISA (ELISA)

2.0-4.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2942206

Product Specific Information

AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT.

Preparation: Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Target Information

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.