Thermo Fisher Scientific PSME2 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

PA1-976 detects recombinant human and mouse proteasome 11S REG beta. This antibody does not detect endogenous levels of proteasome 11S REG beta nor other 11S REG subunits.
PA1-976 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~28 kDa protein representing recombinant human proteasome 11S REG beta.
PA1-976 immunizing peptide corresponds to amino acid residues 1–14 from human proteasome 11S REG beta protein. This peptide (PEP-158) is available for use in neutralization and control experiments.

Target Information

Proteolytic degradation is critical for maintaining appropriate levels of short-lived and regulatory proteins involved in cellular metabolism, stress response, antigen presentation, receptor modulation, ion channels, cell cycle regulation, transcription, and signaling.
The ubiquitin-proteasome pathway degrades most proteins in the eukaryotic cytosol and nucleus, while others are processed via the vacuolar pathway (endosomes, lysosomes, ER).
The 26S proteasome is an ATP-dependent, multisubunit (~31) molecular complex (~2.5 MDa) composed of a 20S core and 19S regulatory subunits. The 19S subunits recognize ubiquitinated proteins and mediate unfolding and translocation into the 20S lumen.
Defects in this pathway are associated with genetic diseases such as cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.