Takara Multiplex PCR Assay Kit Ver. 2

Multiplex PCR allows for the simultaneous amplification of more than one PCR product by the inclusion of multiple primer pairs in a single reaction. This approach saves time, is more economical in the consumption of reagents than standard PCR, and makes it possible to use valuable samples efficiently. Multiplex PCR Assay Kit Ver. 2 includes an enzyme mix for high-speed priming and a buffer (with Mg2+ and dNTPs) optimized for increased specificity of primer annealing.

Multiplex PCR allows for the simultaneous amplification of more than one PCR product by the inclusion of multiple primer pairs in a single reaction. This approach saves time, is more economical in the consumption of reagents than standard PCR, and makes it possible to use valuable samples efficiently. Multiplex PCR Assay Kit Ver. 2 includes an enzyme mix for high-speed priming and a buffer (with Mg2+ and dNTPs) optimized for increased specificity of primer annealing.

When compared to conventional multiplex polymerases, this kit facilitates faster reaction times while requiring less optimization and still achieving highly specific amplification with minimal sequence-specific bias. Depending on the amount of enzyme used and thermal cycling conditions set, it is possible to perform high-throughput multiplex PCR with up to ~200 primer pairs. In addition to multiplexing capabilities, this kit can also be used for standard PCR amplification using otherwise problematic primer pairs that are prone to generating extra bands or smears, or that generally fail to amplify the target fragment.

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