Takara EHEC PCR assay: EHEC (O antigens) PCR Typing Kit
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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
RR133A | Takara RR133A EHEC (O antigens) PCR Typing Kit, 50 Rxns pk | 재고문의 | pk | 0원 | 0원 |
This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.
This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.
Additionally, this kit allows for simultaneous detection of verocytotoxin type 1, verocytotoxin type 2, and the eae gene. This product is designed for analysis of DNA extracted from food samples using the hot alkaline extraction method. After running multiplex PCR, samples are subjected to gel electrophoresis, allowing visualization of O antigens, verocytotoxin, eae gene, and internal control bands.
Note: Associate Professor Atsushi Iguchi of the Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki contributed to the development of this kit.
Find answers to your PCR questions
[Primer design](/learning-centers/pcr/faq/primer-design Primer design
)
Frequently asked questions about primer design for successful PCR.
[Optimization](/learning-centers/pcr/faq/optimization Optimization
)
Frequently asked questions about PCR optimization.
[Troubleshooting](/learning-centers/pcr/faq/troubleshooting Troubleshooting
)
Frequently asked questions about troubleshooting your PCR problems.
[Applications and conditions](/learning-centers/pcr/faq/applications-and-conditions Applications and conditions
)
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
[Shipping, storage, and handling](/learning-centers/pcr/faq/shipping-storage-and-handling Shipping, storage, and handling
)
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
[Avoid DNA contamination in PCR](/about/bioview-blog/tips-and-troubleshooting/avoid-dna-contamination-in-pcr Avoid DNA contamination in PCR
)
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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