Takara EHEC PCR assay: EHEC (O antigens) PCR Typing Kit

This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.

This kit provides a reagent set to allow simple, rapid detection of enterohemorrhagic E. coli (EHEC) by amplification of the verocytotoxin gene using PCR. It has been designed for screening of seven major serotypes within the O-antigen types (O26, O103, O111, O121, O145, O157, and O165). A multiplex PCR enzyme mix, 2X PCR buffer (with Mg2+ and dNTP), primer mix, and controls are supplied with the kit.

Additionally, this kit allows for simultaneous detection of verocytotoxin type 1, verocytotoxin type 2, and the eae gene. This product is designed for analysis of DNA extracted from food samples using the hot alkaline extraction method. After running multiplex PCR, samples are subjected to gel electrophoresis, allowing visualization of O antigens, verocytotoxin, eae gene, and internal control bands.

Note: Associate Professor Atsushi Iguchi of the Department of Animal and Grassland Sciences, Faculty of Agriculture, University of Miyazaki contributed to the development of this kit.

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