Takara O-157 (verocytotoxin genes) one-shot PCR screening

This kit enables simple detection of an enterohemorrhagic E. coli (EHEC) strain, O157:H7 serotype, by specifically amplifying verocytotoxin genes via PCR. One-Shot PCR solution contains all the reagents required to perform this PCR in a 0.2-ml PCR tube. The solution includes Ex Taq DNA polymerase, which gives high yields, high efficiency, and allows highly sensitive detection in less time than conventional Taq Polymerase. All that needs to be added is the sample template DNA.

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PCR learning center

PCR selection guide

PCR FAQs

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Find answers to your PCR questions

[Primer design](/learning-centers/pcr/faq/primer-design Primer design)

Frequently asked questions about primer design for successful PCR.

[Optimization](/learning-centers/pcr/faq/optimization Optimization)

Frequently asked questions about PCR optimization.

[Troubleshooting](/learning-centers/pcr/faq/troubleshooting Troubleshooting)

Frequently asked questions about troubleshooting your PCR problems.

[Applications and conditions](/learning-centers/pcr/faq/applications-and-conditions Applications and conditions)

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

[Shipping, storage, and handling](/learning-centers/pcr/faq/shipping-storage-and-handling Shipping, storage, and handling)

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

[Avoid DNA contamination in PCR](/about/bioview-blog/tips-and-troubleshooting/avoid-dna-contamination-in-pcr Avoid DNA contamination in PCR)

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.