Takara Edman degradation and protein sequencing: Pfu Pyroglutamate Aminopeptidase

Takara Pfu Pyroglutamate Aminopeptidase

Applications

• Removal of pyroglutamic acids from the N-termini of proteins and peptides

Components

Supplied Buffer

• Volume: 1 ml
• Composition: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
• Specific activity comparison:
  • 5.83 U/mg protein at 37°C
  • 12.2 U/mg protein at 50°C
  • 28.3 U/mg protein at 75°C

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Properties

Definition of Activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol pyroglutamate p-nitroanilide at 37°C in 1 minute at pH 7.0.

References

• Hamazume, Y. & Mega, T. Positions of Disulfide Bonds in Riboflavin-Binding Protein of Hen Egg White. J. Biochem. 101, 217–223 (1987).
• Shimada, Y., Sugihara, A., Tominaga, Y., Iizumi, T. & Tsunasawa, S. cDNA molecular cloning of Geotrichum candidum lipase. J. Biochem. 106, 383–388 (1989).

Additional Product Information

Please refer to the product’s Certificate of Analysis for detailed information on storage conditions, components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product and restricted use information available at takarabio.com.