Takara Edman degradation and protein sequencing: Pfu Pyroglutamate Aminopeptidase

More Information

Applications

• Removal of pyroglutamic acids from the N-termini of proteins and peptides

Components

Supplied Buffer

• Volume: 1 ml
• Component: 250 mM sodium phosphate buffer (pH 7.0) containing 50 mM DTT and 5 mM EDTA
• Comparison of specific activities across different temperatures: 5.83 U/mg protein at 37°C showed activities of 12.2 U/mg protein or 28.3 U/mg protein at 50°C or 75°C, respectively.

Source

Recombinant Escherichia coli encoding the Pyrococcus furiosus pyroglutamate aminopeptidase gene.

Properties

Definition of activity

One unit of enzyme activity corresponds to the amount required to hydrolyze 1 µmol pyroglutamate p-nitroanilide at 37°C in 1 minute at pH 7.0.

References

Hamazume, Y. & Mega, T. Positions of Disulfide Bonds in Riboflavin-Binding Protein of Hen Egg White. J. Biochem 101, 217–223 (1987).

Shimada, Y., Sugihara, a, Tominaga, Y., Iizumi, T. & Tsunasawa, S. cDNA molecular cloning of Geotrichum candidum lipase. J. Biochem. 106, 383–388 (1989).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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