Thermo Fisher Scientific Phospho-DOK1/DOK2 (Tyr362, Tyr337) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000
• Publications: [References not provided]

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to a phosphotyrosine peptide before affinity purification using phospho-Dok1 (Tyr-362) peptide.
The purified antibody detects a band at 62 kDa corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after alkaline phosphatase treatment.
Similar to Dok1 (Ser-450) antibody, this antibody also detects an 80 kDa band that has not been identified.

Target Information

Doks are a family of adaptor proteins including six members (Dok1–Dok6) with:

• N-terminal pleckstrin homology domain
• Central phosphotyrosine binding domain
• C-terminal region containing multiple tyrosine residues

When phosphorylated, these tyrosines serve as docking sites for SH2 domain-containing proteins.
Dok1 (p62dok) binds Ras-GAP, Nck, and Csk. Tyr-362 (Tyr-361 mouse) is phosphorylated by c-Abl, required for Nck binding, and may be critical for filopodia formation during fibroblast spreading on fibronectin.
Dok1 activity is also regulated by serine phosphorylation. IkappaB Kinase beta phosphorylates several serine sites including Ser-450 in vitro, and TNFα, IL-1, and radiation treatment lead to phosphorylation of Ser-443, Ser-446, and Ser-450 in vivo.
Phosphorylation of these serine sites may be required for Dok-mediated inhibition of MAPK signaling and stimulation of cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.