Thermo Fisher Scientific Phospho-DOK1 (Ser450) Polyclonal Antibody

Applications

• Western Blot (WB): 1:100

Product Specifications

Product Specific Information

This antibody was cross-adsorbed to an unphosphorylated Dok1 (Ser-450) peptide before affinity purification using phospho-Dok1 (Ser-450) peptide.
The purified antibody detects a band at 62 kDa corresponding to Dok1 in western blots of human Jurkat cells, but does not detect this band after lambda phosphatase treatment.
Similar to Dok1 (Tyr-362) antibody, this antibody also detects an 80 kDa band that has not been identified.

Target Information

DOK1 (P62dok, Docking protein 1) is a mainly cytoplasmic adaptor protein that down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and migration.
DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein.
Phosphorylation on tyrosine residues by the insulin receptor kinase results in negative regulation of the insulin signaling pathway.
DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.