Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 13 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• PE
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

This MILAN8R monoclonal antibody recognizes phosphorylated ERK1/2 (Thr202/Tyr204) in human and mouse cells. ERK1/2 are mitogen-activated protein kinases (MAPKs) involved in cellular proliferation, differentiation, motility, and survival. Activation occurs via MEK1/2-mediated phosphorylation, and downstream targets include p90RSK and Elk-1. Dysregulation of ERK signaling is common in cancer.

• Specificity: Confirmed by ELISA, flow cytometry, and western blotting
• Applications Reported: Intracellular staining followed by flow cytometry
• Applications Tested: Pre-titrated for intracellular staining of stimulated human PBMCs or mouse splenocytes
• Recommended Use: 5 µL (0.06 µg)/test in 100 µL final volume; cell number 10⁵–10⁸/test

Protocols:
Use Protocol C (Fixation/Methanol). Protocols A and B are not suitable. Refer to Staining Intracellular Antigens for Flow Cytometry Protocol for details.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm, excited by 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable). Ensure instrument compatibility.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57).

Filtration:
Post-manufacturing filtered (0.2 µm).

Target Information

ERK1 and ERK2 regulate meiosis, mitosis, and postmitotic functions in differentiated cells. They are activated by various stimuli including growth factors, cytokines, and GPCR ligands. Activation involves Ras–Raf–MEK–ERK signaling cascade, leading to phosphorylation of cytoplasmic and nuclear targets such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지