Thermo Fisher Scientific Lamin A+C Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Lamins are structural protein components of the nuclear lamina, which contains three members: Lamin A, B, and C in mammalian cells. Lamin A and Lamin C are generated by alternative splicing from the same gene and share complete identity for the first 566 amino acids. Lamin A interacts with transcription factor SREBP1 via its C-terminal domain. Lamin A/C deficiency is associated with defective nuclear mechanics and impaired transcriptional activation. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation.

This antibody (Rabbit Anti-Lamin A+C Polyclonal Antibody) is developed in rabbit using a KLH-coupled synthetic peptide within residues 400–450 of human Lamin A (Swiss Prot: P02545).

Target Information

Lamins are intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins exist in multiple cell types as three major forms (A, B, and C). Lamins A and C are alternatively spliced products of the LMNA gene.

Mutations in the LMNA gene are associated with various disorders, including:

• Emery-Dreifuss muscular dystrophy
• Dunnigan-type familial partial lipodystrophy (FPLD)
• Limb-girdle muscular dystrophy (LGMD1B)
• Dilated cardiomyopathy (CMD1A)
• Axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1)
• Mandibuloacral dysplasia (MAD)

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.