Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (1F4E3), THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1.0 µg/mL

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 22 publications 22 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Pig

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1F4E3

Immunogen

A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Tubulin,uniProtId:P0DPH7-1,ncbiNodeId:9606,antigenRange:427-441,antigenLength:450,antigenImageFileName:A01410-100_alpha_Tubulin_P0DPH7-1_House_mouse.svg,antigenImageFileNamePDP:A01410-100_alpha_Tubulin_P0DPH7-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to alpha-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.