Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (1F4E3), THE
Alpha Tubulin 단백질을 인식하는 Mouse monoclonal antibody로, WB, ICC/IF, ELISA 등에 적합합니다. 다양한 종(인간, 생쥐 등)에 반응하며, Lyophilized 형태로 안정적 보관이 가능합니다. 미세소관 연구 및 세포골격 분석에 유용합니다.
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- A01410100
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific alpha Tubulin Monoclonal Antibody (1F4E3), THE
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1.0 µg/mL |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| ELISA | 0.05–0.2 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Non-human primate, Pig |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F4E3 |
| Immunogen | Synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
Tubulin is a member of a small family of globular proteins, primarily alpha-tubulin and beta-tubulin, which make up microtubules—essential structural components found in nearly all eukaryotic cells. Monoclonal antibodies to alpha-tubulin serve as reliable housekeeping protein markers and are valuable tools for accurate interpretation of western blot analyses.
Target Information
Alpha Tubulin forms a heterodimer with beta Tubulin, each approximately 55 kDa, constituting the basic building block of microtubules. These intracellular filamentous structures are essential in nearly all eukaryotic cells, contributing to cell structure, transport, and mitosis.
Microtubules are composed mainly of alpha and beta tubulins, while gamma tubulin is crucial for microtubule nucleation. Multiple alpha and beta tubulin genes are highly conserved across species. The alpha tubulin gene shows high similarity to mouse and rat Tuba1 genes, with expression predominantly in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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