Thermo Fisher Scientific TAP-tag Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide CSSGALDYDIPTTASENLYFQ conjugated to KLH.

Conjugate

HRP HRP HRP

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The TAP (tandem affinity purification) tag allows rapid purification of complexes from a relatively small number of cells without prior knowledge about the composition, activity, or function of the complex. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The Rabbit anti-TAP tag consists a 21 residue peptide, CSSGALDYDIPTTASENLYFQ, derived from c-terminal of the TAP construct after TEV cleavage.GenScript Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain TAP tag.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the TAP tag (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.