Thermo Fisher Scientific TAP-tag Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 0.5–1 µg/mL

ELISA

• Tested Dilution: 0.05–0.2 µg/mL

Miscellaneous (PubMed)

• Publications: View 2 publications

Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The TAP (tandem affinity purification) tag allows rapid purification of complexes from a relatively small number of cells without prior knowledge about the composition, activity, or function of the complex.
Combined with mass spectrometry, the TAP strategy allows identification of proteins interacting with a given target protein.

The Rabbit anti-TAP tag consists of a 21-residue peptide, CSSGALDYDIPTTASENLYFQ, derived from the C-terminal of the TAP construct after TEV cleavage.
GenScript Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and conjugated to horseradish peroxidase.
This antibody is suitable for detecting fusion proteins that contain TAP tag.

Target Information

Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are not available.
Tandem affinity purification (TAP) is an affinity purification method for isolating TAP-tagged proteins together with associated proteins.
The TAP tag typically includes a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV) cleavage site, and Protein A fused to the protein of interest.
This technique is useful for analyzing in vivo protein interactions.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.