Takara PrimeSTAR GXL Premix Fast, Dye plus

PrimeSTAR GXL Premix Fast, Dye plus is a convenient 2X PCR master mix containing PrimeSTAR GXL DNA Polymerase, a blue gel-loading dye, and a density reagent for easy processing. PrimeSTAR GXL polymerase is a hot-start, high-fidelity enzyme optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.

PrimeSTAR GXL Premix Fast, Dye plus is a convenient 2X PCR master mix containing PrimeSTAR GXL DNA Polymerase, a blue gel-loading dye, and a density reagent for easy processing. PrimeSTAR GXL polymerase is a hot-start, high-fidelity enzyme optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.

PrimeSTAR GXL Premix Fast, Dye plus enables you to save time and simplify your amplification workflow by incorporating unsurpassed processivity and sensitivity. Simply add primers and template, and run the PCR. Additionally, you can rapidly screen your amplicons by loading them onto an agarose gel immediately after amplification or digesting directly with restriction enzymes, without purification.

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Overview

• Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA), or 13.5 kb (human cDNA)
• Fast PCR extension (5 sec/kb)
• Antibody-mediated hot-start formulation
• High tolerance for GC-rich templates as well as the presence of excess nucleic acid templates

More Information

Applications:

• Cloning (available in combination with In-Fusion reagent)
• Sequencing/Library Prep
• Multiplex HLA typing
• Any difficult or variable samples
• Antibody engineering
• Reverse genetics system for SARS-CoV-2 using CPER method

Termini of PCR products

• PrimeSTAR GXL DNA Polymerase generates products with blunt ends. It is suitable for use in conjunction with blunt-ended cloning systems.

Recommended maximum amplicon length

• Up to 30 kb (human genomic DNA template)
• Up to 40 kb (lambda DNA template)
• Up to 13.5 kb (human cDNA template)

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.