Takara PCR Human Papillomavirus Typing Set

This kit is designed for the identification of a broad range of human papillomavirus (HPV) types using HPV type-specific PCR. This set uses two pairs of consensus primers (HPVpU-1M/HPVpU-2R and HPVpU-31B/HPVpU-2R), which are designed to amplify the E6 and E7 homologous regions of HPV (228–268 bp). Included in this set are specific primers and control templates.

This kit is designed for the identification of a broad range of human papillomavirus (HPV) types using HPV type-specific PCR. This set uses two pairs of consensus primers (HPVpU-1M/HPVpU-2R and HPVpU-31B/HPVpU-2R), which are designed to amplify the E6 and E7 homologous regions of HPV (228–268 bp). Included in this set are specific primers and control templates.

Malignant types (HPV16, 18, 33, 52b and 58) types are amplified using the HPVpU-1M/HPVpU-2R primer pair; benign tpes (HPV6 and 11) types are amplified using the HPVpU-31B/HPVpU-2R primer pair. Digestion of PCR products by restriction enzymes AccI, AfaI, AvaI, AvaII, and BglII (available as Enzyme Set A, Cat. # 6604) followed by agarose gel electrophoresis identifies the resident HPV types. This PCR HPV Typing Set is optimized for use with TaKaRa Taq DNA Polymerase, which can be purchased separately.

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[Primer design](/learning-centers/pcr/faq/primer-design Primer design)

Frequently asked questions about primer design for successful PCR.

[Optimization](/learning-centers/pcr/faq/optimization Optimization)

Frequently asked questions about PCR optimization.

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[Avoid DNA contamination in PCR](/about/bioview-blog/tips-and-troubleshooting/avoid-dna-contamination-in-pcr Avoid DNA contamination in PCR)

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.