| 상품 옵션 정보 | ||||||||
|---|---|---|---|---|---|---|---|---|
| 카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
| 5333 | - | Takara 5333 EpiScope® Nucleosome Preparation Kit, 50 Rxns pk | 재고문의 | pk | 531,000원 | - | 584,100원 | |
Overview
- Enables detailed analysis of nucleosome positioning
- Prepared DNA consists primarily of mononucleosomes
More Information
Applications
- Preparation of nucleosomal DNA from 0.5–2 x 106 mammalian cultured cells
- Nucleosome positioning with regards to DNA methylation analysis
Components
| Package 1 | |
|---|---|
| Micrococcal Nuclease (20 units/μl) | 25 ml × 2 |
| Micrococcal Nuclease Buffer (10X) | 1 ml |
| RNase A (20 mg/ml) | 100 μl |
| 0.5 M EDTA | 100 μl |
| Proteinase K (20 mg/ml) | 200 μl |
| LINE-1 qPCR Primer (10 μM each)* | 50 μl |
| Package 2 | |
|---|---|
| Cytoplasmic Lysis Buffer | 50 μl |
| Protease Inhibitor Cocktail (100X) | 550 μl |
*This component is to be used as a reference for making corrections to the amounts of genomic DNA in relative quantification analyses by real-time PCR. It is a primer for human genomic DNA detection and cannot be used with other species.
Storage
Package 1: –20℃
Package 2: 4℃
References
Clark, D. J. Nucleosome positioning, nucleosome spacing and the nucleosome code. J. Biomol. Struct. Dyn. 27, 781–93 (2010).
Dutta, A. & Workman, J. L. Nucleosome Positioning: Multiple Mechanisms toward a Unifying Goal. Mol. Cell 48, 1–2 (2012).
Additional product information
Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
배송/결제/교환/반품 안내
배송 정보
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