Thermo Fisher Scientific Mouse GM-CSF Carrier-Free Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Product Specifications

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: Mouse Granulocyte/Macrophage Colony Stimulating Factor (GM-CSF) is an ~14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF alpha, IL-2, IL-1, and IFN gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported: Mouse GM-CSF Recombinant Protein Carrier-Free has been reported for use in cytokine bioassays.

Applications Tested: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg.

Source: E. coli expressed mature mouse GM-CSF accession # NM_009969.

Bioactivity: The ED50 of this protein, as measured by MC/9 cell proliferation assay, is less than or equal to 650 pg/mL. This corresponds to a specific activity of greater than or equal to 1.5 x 10e6 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.