Thermo Fisher Scientific Mouse IFN-alpha 2 (Carrier-Free) Recombinant Protein, eBioscience
Carrier-free recombinant Mouse IFN-alpha 2 protein for ELISA and bioassay applications. Expressed in E. coli, >98% purity, low endotoxin (<0.01 ng/µg). Active in antiviral and antiproliferative assays. Supplied as liquid, 0.5 mg/mL, stored at -80°C.
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- 34-8312-85
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- Protein
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- pk
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Thermo Fisher Scientific Mouse IFN-alpha 2 (Carrier-Free) Recombinant Protein, eBioscience
Applications and Tested Dilution
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Mouse |
| Expression System | E. coli |
| Amino Acid Sequence | Mouse IFN-alpha 2, amino acids Cys24–Glu190 (Accession # NM_010503) |
| Molecular Weight | 19.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE |
| Endotoxin Concentration | <0.01 ng/µg |
| Activity | ED50 ~0.1 ng/mL (inhibition of EMC virus cytopathic effect on L929 cells), specific activity 1×10⁷ Units/mg |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | 50 mM sodium acetate, pH 5.0, with 0.1 M NaCl |
| Contains | No preservative |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
Product Specific Information
Description:
IFN-alpha2 is a type I interferon, also known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. It is one of at least 23 known variants of IFN-alpha, with molecular masses between 19–26 kDa and lengths of 156–172 amino acids. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and various other cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular-weight substances (e.g., n-butyrate, 5-bromodeoxyuridine). IFN-alpha2 exhibits antiviral, antiparasitic, and antiproliferative activities.
Applications Reported:
Useful for bioassay and ELISA.
Applications Tested:
Inhibition of cytopathic effect of EMC virus on L929 cells (ED50 ~0.1 ng/mL; specific activity 1×10⁷ Units/mg).
Source:
E. coli expressed amino acids Cys24–Glu190 of mature mouse IFN-alpha2 (Accession # NM_010503).
Bioactivity:
Confirmed by inhibition of EMC virus cytopathic effect on L929 cells.
Endotoxin:
<0.01 ng/µg cytokine (LAL assay).
Purity:
98% (SDS-PAGE).
Molecular Weight:
Predicted molecular mass 19,361 Da.
Non-reduced protein migrates as 16 kDa; DTT-reduced protein migrates as 18 kDa on SDS-PAGE.
Storage and Handling:
Quick-spin vial prior to opening for best recovery. Use in sterile environment.
Target Information
IFN-alpha (Interferon alpha) is a Type I interferon, a family of structurally related, species-specific proteins found in vertebrates. Interaction with its receptor activates signaling pathways involved in innate immune responses against viral infection. In humans, the IFN-alpha gene is located on chromosome 9. Type I interferons include 14 alpha isoforms and single beta, omega, epsilon, and kappa isoforms. Homologous molecules exist in many species, including rats, mice, birds, reptiles, and fish. IFN-alpha is mainly produced by plasmacytoid dendritic cells and plays a key role in antiviral defense and modulation of adaptive immunity. Dysfunction of IFN-alpha is associated with diseases such as Hepatitis C and Hepatitis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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