Thermo Fisher Scientific PSD-95 Monoclonal Antibody (6G6), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

Immunohistochemistry (IHC)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6G6

Immunogen

Recombinant rat PSD-95

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G, Protein A

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.2, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931593

Product Specific Information

1 µg/mL was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.|Detects approximately 100kDa. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at approximately 75kDa and 50kDa in rat and mouse samples.

Target Information

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.