Thermo Fisher Scientific PSD-95 Monoclonal Antibody (7E3), FITC
PSD-95 단백질 검출용 FITC 결합 마우스 단클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, 신경 시냅스 단백질 연구에 유용. 4°C 암소 보관, Protein G 정제 제품.
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Thermo Fisher Scientific PSD-95 Monoclonal Antibody (7E3), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E3 |
| Immunogen | Recombinant rat PSD-95 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS / 9.1 mM sodium bicarbonate with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931595 |
Additional Formats
Product Specific Information
1 µg/mL was sufficient for detection of PSD-95 on 20 µg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary. Detects approximately 100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at approximately 75 kDa and 50 kDa in rat and mouse samples.
Target Information
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is a membrane-associated protein located in the postsynaptic density of forebrain neurons and presynaptic structures in the cerebellum. PSD-95 contains three PDZ domains, one SH3 domain, and one Guanylate Kinase (GuK) domain. It plays a key role in clustering NMDA receptors and Shaker-type potassium channels at the synaptic membrane in CNS neurons. PSD-95 interacts with proteins containing a C-terminal T/SXV motif and associates with neuronal nitric oxide synthase (nNOS) through PDZ-PDZ interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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