Thermo Fisher Scientific alpha Dystroglycan Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:20-1:100

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 776-895 of human DAG1 (NP_0011711081) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Dystroglycan,uniProtId:Q14118-1,ncbiNodeId:9606,antigenRange:776-895,antigenLength:895,antigenImageFileName:PA5-116504_alpha_Dystroglycan_Q14118-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116504_alpha_Dystroglycan_Q14118-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.87 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901135

Product Specific Information

Positive Samples: HepG2, U2OS, SW480, HeLa, mouse skeletal muscle, mouse lung, mouse heart, rat liver, rat lung

Immunogen sequence: RKKRKGKLTL EDQATFIKKG VPIIFADELD DSKPPPSSSM PLILQEEKAP LPPPEYPNQS VPETTPLNQD TMGEYTPLRD EDPNAPPYQP PPPFTAPMEG KGSRPKNMTP YRSPPPYVPP

Target Information

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.