Thermo Fisher Scientific alpha Dystroglycan Polyclonal Antibody
Thermo Fisher Scientific의 alpha Dystroglycan Polyclonal Antibody는 마우스 및 랫트 시료에 반응하며, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. Rabbit IgG 기반의 비결합 액상 항체로, 고순도의 친화 크로마토그래피 정제 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 776–895 of human DAG1 (NP_001171108.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.87 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901135 |
Product Specific Information
- Positive Samples: HepG2, U2OS, SW480, HeLa, mouse skeletal muscle, mouse lung, mouse heart, rat liver, rat lung
- Immunogen Sequence:
RKKRKGKLTL EDQATFIKKG VPIIFADELD DSKPPPSSSM PLILQEEKAP LPPPEYPNQS VPETTPLNQD TMGEYTPLRD EDPNAPPYQP PPPFTAPMEG KGSRPKNMTP YRSPPPYVPP
Target Information
Dystroglycan is a laminin-binding component of the dystrophin-glycoprotein complex, providing a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of mutation in autosomal recessive muscular dystrophies. The reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, making muscle fibers more susceptible to necrosis. It also functions as a dual receptor for agrin and laminin-2 in Schwann cell membranes. Muscle and nonmuscle isoforms differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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