Takara TB Green Premix DimerEraser (Perfect Real Time)

Overview

• Quick and accurate detection and quantification of target gene through real time PCR
• Easy-to-use 2X premix reagent including TB Green: Ready to perform real-time PCR in the presence of a fluorescent intercalator. Just add PCR primers, template, and dH2O to start the reaction.
• High amplification efficiency and highly sensitive detection: this product utilizes an enzyme for hot start, Takara Ex Taq HS. As this enzyme buffer system is optimized for real-time PCR, this product offers highly efficient amplification and highly sensitive detection. Moreover, the addition of the accessory protein strongly suppresses mis-priming of primers during PCR reaction and nonspecific amplification, including primer-dimer amplification.

More Information

Applications

• For real-time qPCR using TB Green

Applicable real-time PCR instruments

• ABI PRISM 7000, Applied Biosystems 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System (Applied Biosystems)
• LightCycler (Roche Diagnostics)
• Thermal Cycler Dice Real Time System (TAKARA BIO) (Note: This instrument is not available in the U.S. or Europe.)
• Smart Cycler System/Smart Cycler II System (Cepheid)

Needed, but not included in the kit:

• Thermal cycler for real time PCR
• Reaction tube or plate for real time PCR
• PCR primer
• dH2O
• Micropipettors and tips (autoclaved prior to use)

Notes

Takara Ex Taq HS used in this product is an enzyme for hot-start PCR utilizing a Taq antibody.

The initial denaturation step prior to PCR should be 95°C for 30 sec. It does not require the activation step of heating at 95°C for 10–15 min prior to the PCR.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.