Takara Acylamino-acid-releasing enzyme

More Information

Applications

• Preparation of proteins and peptides for N-terminal amino acid sequencing

Components

Volume: 1 ml
Component: 250 nM sodium phosphate, pH 7.2

Source

Porcine liver, lyophilized

Purity

Homogeneous on SDS-PAGE

Storage

–20°C (under dry conditions). Store reconstituted solution at 4°C.

Properties

• Molecular weight: 360,000 (gel filtration chromatography; 75,000 (SDS-PAGE)
• Optimum pH: 7.2–7.6 (Ac-Met-Ala)
• Km value: 0.41 mM (Ac-Met-Ala)
• Inhibitors: p-chloromercuribenzonate (PCMB; diisopropyl fluorophosphate (DFP))

Note: This enzyme cannot react with peptides having Ac-Pro-X-, Ac-Y-Pro-, Ac-Trip-X-, Ac-Asp-X-, or Ac-Glu-X- at their N termini. This enzyme also cannot react with peptides that have Ac-Met-Asp- at their N termini.

Definition of activity

One unit of enzyme activity corresponds to the amount required to produce 1 µmol of L-Ala in 1 minute at 37°C from Ac-Met-Ala at pH 7.2.

Volume

0.5 U/vial (contains about 9 mg of sucrose in each vial)

Systematic name

N-Acylamino-peptide hydrolase

Enzyme code

3.4.19.1

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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