
Takara Acylamino-acid-releasing enzyme
단백질 및 펩타이드의 N-말단 아미노산 서열 분석용 효소. 돼지 간 유래 동결건조 효소로 SDS-PAGE에서 균질함. 최적 pH 7.2–7.6, 분자량 약 360,000. 0.5 U/vial, -20°C 건조 조건에서 보관.
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Takara Acylamino-acid-releasing Enzyme
Applications
- Preparation of proteins and peptides for N-terminal amino acid sequencing
Components
| 항목 | 내용 |
|---|---|
| Volume | 1 ml |
| Buffer Composition | 250 nM sodium phosphate, pH 7.2 |
Source
Porcine liver, lyophilized
Purity
Homogeneous on SDS-PAGE
Storage
- –20°C (under dry conditions)
- Store reconstituted solution at 4°C
Properties
| 항목 | 내용 |
|---|---|
| Molecular weight | 360,000 (gel filtration chromatography); 75,000 (SDS-PAGE) |
| Optimum pH | 7.2–7.6 (Ac-Met-Ala) |
| Km value | 0.41 mM (Ac-Met-Ala) |
| Inhibitors | p-chloromercuribenzonate (PCMB); diisopropyl fluorophosphate (DFP) |
Note:
This enzyme cannot react with peptides having Ac-Pro-X-, Ac-Y-Pro-, Ac-Trp-X-, Ac-Asp-X-, or Ac-Glu-X- at their N termini.
It also cannot react with peptides that have Ac-Met-Asp- at their N termini.
Definition of Activity
One unit of enzyme activity corresponds to the amount required to produce 1 µmol of L-Ala in 1 minute at 37°C from Ac-Met-Ala at pH 7.2.
Volume
0.5 U/vial (contains about 9 mg of sucrose per vial)
Systematic Name
N-Acylamino-peptide hydrolase
Enzyme Code
EC 3.4.19.1
Additional Product Information
Please refer to the product’s Certificate of Analysis for detailed information about storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates in the U.S. and/or other countries.
Additional product and intellectual property information available at takarabio.com.
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