Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), Alexa Fluor 660, eBioscience
Ki-67 단백질을 인식하는 SolA15 단클론 항체로, 세포 증식 지표 분석에 사용됩니다. Alexa Fluor 660 형광 표지로 유세포 분석 및 면역염색에 적합합니다. 마우스, 랫드, 인간 등 다양한 종에 반응하며, 4°C 보관 및 암조건에서 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 0.25 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | Alexa Fluor™ 660 |
| Excitation / Emission Max | 663 / 691 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896286 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- Alexa Fluor Plus 594
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- FITC
- PE
- PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein present during all active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0). Ki-67 serves as a marker for determining the growth fraction of cell populations and is used in tumor proliferation studies.
The SolA15 antibody also recognizes human, non-human primate, and canine Ki-67.
It has been validated for intracellular staining followed by flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Recommended usage: ≤0.25 µg/test (100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Optimal titration is recommended for best performance.
Fluorophore information:
Alexa Fluor 660 emits at 690 nm and is suitable for use on spectral cytometers.
- Excitation: 662 nm
- Emission: 690 nm
- Laser: Red Laser
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus via its FHA domain.
Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis. It is valuable in determining tumor growth fractions and correlates with tumor grade and prognosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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