Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Super Bright 436, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/Test
• Publications: View 9 publications

Product Specifications

Additional Formats

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein coupled chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts, with CXCR3 knockout mice showing compromised allograft rejection. Expression is found on NK cells, subsets of T lymphocytes, Tregs, and Th1-polarized cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤ 0.5 µg/test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Fluorochrome Information:
Super Bright 436 is excited with the 405 nm violet laser and emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

Staining Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily, characterized by a single amino acid separating the first two conserved cysteine residues. Binding induces cellular responses such as integrin activation, cytoskeletal changes, and chemotactic migration. Expression is prominent in effector/memory T cells and inflamed tissues, implicating CXCR3 in inflammatory cell recruitment. It is a potential target for small-molecule antagonists in inflammatory disease treatment. Multiple transcript variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지