Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 436, eBioscience
CD171 (L1CAM) 인식하는 eBio5G3 모노클론 항체로, Super Bright 436 형광체 결합. 인간 시료에 반응하며 Flow Cytometry에 최적화됨. 신경세포 접착 및 종양 연구용으로 적합. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 62-1719-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 436, eBioscience
Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 2 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG2a |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5G3 (5G3) |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762525 |
Additional Formats
Product Specific Information
The monoclonal antibody eBio5G3 recognizes CD171 (L1CAM), a neural cell adhesion molecule belonging to the Ig superfamily. It mediates homotypic and heterotypic cell-cell interactions involved in neuronal myelination, neurite outgrowth, and regeneration. CD171 is expressed on monocytes, dendritic cells, subsets of lymphocytes, neuronal tissues, and certain tumor cell lines. Its expression is associated with tumor progression. The eBio5G3 epitope is located in the amino-terminal Ig-like domain.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested on Panc-1 tumor cell line at 5 µL (0.5 µg)/test. A test is defined as staining in 100 µL volume; cell number may range 10⁵–10⁸ cells/test.
Super Bright 436 is excited at 405 nm (violet laser) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to reduce non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
L1CAM/CD171 is an axonal glycoprotein of the immunoglobulin superfamily, composed of immunoglobulin-like and fibronectin type III-like domains. It plays a key role in neuronal development, migration, and differentiation. Mutations in L1CAM cause X-linked neurological syndromes (CRASH: corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, hydrocephalus). Alternative splicing of a neuron-specific exon is functionally relevant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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