Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 436, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio5G3 (5G3)

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

View additional formats

• Unconjugated
• APC
• Biotin
• PE
• Super Bright 600
• Request custom conjugation

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762525

Product Specific Information

Description: The monoclonal antibody eBio5G3 recognizes CD171 also known as neural cell adhesion molecule L1. CD171 is a member of the Ig superfamily containing 6 extracellular Ig domains and five fibronectin type III-like repeats. CD171 has been shown to function as a cell adhesion molecule mediating homotypic and heterotypic cell-cell interactions in neuronal myelination, neurite outgrowth and regeneration. Expression of CD171 has been found on monocytes and mature monocytic-derived and follicular DCs, a minor subset of lymphocytes in addition to that found on neuronal tissue and some tumor cells lines. Expression of CD171 on tumors is thought to contribute to tumor progression. Epitope of eBio5G3 is in amino-terminal Ig-like domain.

Applications Reported: This eBio5G3 (5G3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5G3 (5G3) antibody has been pre-titrated and tested by flow cytometric analysis of tumor cell line Panc-1. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.