Thermo Fisher Scientific BUBR1 Monoclonal Antibody (8G1), DyLight 488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

Assay-Dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8G1

Immunogen

A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BUBR1,uniProtId:O60566-1,ncbiNodeId:9606,antigenRange:1-350,antigenLength:1050,antigenImageFileName:MA5-16037_BUBR1_O60566-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16037_BUBR1_O60566-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

View additional formats

• Unconjugated
• Biotin
• HRP
• Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

0.6 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153228

Target Information

BUBR1 is a member of the Ser/Thr protein kinase family. It is a component of the mitotic checkpoint that delays anaphase until all chromosome are properly attached to the mitotic spindle. Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.