Thermo Fisher Scientific BUBR1 Monoclonal Antibody (8G1), DyLight 488
Thermo Fisher Scientific의 BUBR1 Monoclonal Antibody (8G1), DyLight 488은 인간 BUBR1 단백질을 표적하는 형광 표지 항체입니다. Western blot, ICC/IF, Flow cytometry에 사용 가능하며, DyLight 488 형광(492/519 nm)을 제공합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8G1 |
| Immunogen | A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1–350 of human BUBR1) |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153228 |
Additional Formats
Target Information
BUBR1 is a member of the Ser/Thr protein kinase family and a component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. Defects in spindle checkpoints can lead to genetic instability and malignant transformations. Human cells express two related checkpoint kinases derived from the BUB1 gene: hBUBR1 and hBUB1. These kinases monitor kinetochore-microtubule interactions and are essential for normal mitotic progression. BUBR1 has been detected in human cancers and identified as a binding partner of BCSG1, which may accelerate breast cancer progression by compromising mitotic checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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