Takara E. coli electrocompetent cells

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Cell density

> 1 × 1010 bacteria/ml

Purity

When inoculating 50 µl of E. coli electrocompetent cells on LB-agar medium containing ampicillin at 100 µg/ml, colonies are not formed.

Genotype

• E. coli HB101: supE_44, Δ(_mcrC-mrr), _recA_13, _ara_-14, _proA_2, _lacY_1, _galK_2, _rpsL_20, _xyl_-5, _mtl_-1, _leuB_6, _thi_-1

• E. coli JM109: recA_1, _endA_1, _gyrA_96, _thi_-1, _hsdR_17(rK-mk+), e14–(_mcrA_–), _supE_44, _relA_1, Δ(_lac-proAB)/F` [traD_36, _proAB+, _lac I_q, _lacZ_ΔM15]

• E. coli CJ236: _dut_1, _ung_1, _thi_-1, _relA_1/pCJ105(F` _cam_r)

• E. coli BMH71-18 mutS: Δ(lac-proAB), supE, thi_-1, _mutS_215:: Tn_10 (tet_r)/F` [_traD_36, _proAB+, _lac I_q, _lacZ_ΔM15]

• E. coli MV1184: ara, Δ(lac-proAB), rpsL, thi ( φ80_lacZ_ΔM15), Δ(srl-recA)306:: Tn_10_ (tet_r)/ F`[_traD_36, _proAB+, _lac I_q, _lacZ_ΔM15]

• E. coli TH2: _supE_44, _hsdS_20(rB– mB–), _recA_13, _ara_-14, _proA_2, _lacY_1, _galK_2, _rpsL_20, _xyl_-5, _mtl_-1, _thi_-1, _trpR_624

• E. coli DH5α: F–, φ80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, deo_R, _recA_1, _endA_1, _hsdR_17(rK–, mK+), _phoA, _supE_44, λ–, _thi_-1, _gyrA_96, _relA_1

• E. coli HST08 Premium: F-, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, λ80d_lacZ_ΔM15, Δ(lacZYA_-_argF)U169, Δ(mrr_-_hsdRMS_-_mcrBC), ΔmcrA, λ-

Transformation efficiency

Stability of F` plasmid, confirmation of α-complementation:

• *E. coli JM109, E. coli BMH71-18 mutS, E. coli MV1184
  *Less than 1% of white colonies appeared when transformation with a plasmid vector of pUC19 (with JM109) or pUC119 (with BMH71-18 mutS or MV1184) followed by plating on L-agar medium containing 100 µg/ml ampicillin, 0.2 mM IPTG, and 40 µg/ml X-Gal.

• ** E. coli CJ236
  **Strains lacking F` plasmid (pCJ105) do not grow by using a plate containing chloramphenicol at 30 µg/ml.

• E. coli DH5αBlue colony appeared when pUC19 DNA was transformed and plated the transformants on L-agar medium containing 100 µg/ml of ampicillin and 40 µg/ml of X-Gal.

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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