Takara E. coli electrocompetent cells
고효율 E. coli 전기적 형질전환 세포로 다양한 클로닝 및 플라스미드 증폭에 최적화됨. 여러 균주(HB101, JM109, DH5α 등) 제공으로 다양한 실험 목적에 대응. 높은 형질전환 효율과 F' 플라스미드 안정성 보장. 연구용으로만 사용 가능.
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Takara E. coli Electrocompetent Cells
Cell Density
1 × 10¹⁰ bacteria/ml
Purity
When inoculating 50 µl of E. coli electrocompetent cells on LB-agar medium containing ampicillin at 100 µg/ml, colonies are not formed.
Genotype Information
| Strain | Genotype |
|---|---|
| E. coli HB101 | supE44, Δ(mcrC-mrr), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, leuB6, thi-1 |
| E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(rK-mk+), e14–(mcrA–), supE44, relA1, Δ(lac-proAB)/F` [traD36, proAB+, lacIq, lacZΔM15] |
| E. coli CJ236 | dut1, ung1, thi-1, relA1/pCJ105(F` camr) |
| E. coli BMH71-18 mutS | Δ(lac-proAB), supE, thi-1, mutS215::Tn10 (tetr)/F` [traD36, proAB+, lacIq, lacZΔM15] |
| E. coli MV1184 | ara, Δ(lac-proAB), rpsL, thi (φ80_lacZ_ΔM15), Δ(srl-recA)306::Tn10 (tetr)/F` [traD36, proAB+, lacIq, lacZΔM15] |
| E. coli TH2 | supE44, hsdS20(rB– mB–), recA13, ara-14, proA2, lacY1, galK2, rpsL20, xyl-5, mtl-1, thi-1, trpR624 |
| E. coli DH5α | F–, φ80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK–, mK+), phoA, supE44, λ–, thi-1, gyrA96, relA1 |
| E. coli HST08 Premium | F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, λ80d_lacZ_ΔM15, Δ(lacZYA-argF)U169, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, λ– |
Transformation Efficiency
| Strain | Efficiency (cfu/µg DNA) | Test Plasmid |
|---|---|---|
| HB101 | >5 × 10⁸ | pBR322 |
| JM109 | >1 × 10⁹ | pUC19 |
| MV1184 | >1 × 10⁸ | pUC119 |
| DH5α | >1 × 10⁹ | pUC19 |
| HST08 Premium | >1 × 10⁹ | pUC19 |
Stability of F` Plasmid and α-Complementation
E. coli JM109, BMH71-18 mutS, MV1184
Less than 1% of white colonies appeared when transformed with pUC19 (JM109) or pUC119 (BMH71-18 mutS, MV1184) and plated on L-agar with 100 µg/ml ampicillin, 0.2 mM IPTG, and 40 µg/ml X-Gal.E. coli CJ236
Strains lacking F` plasmid (pCJ105) do not grow on plates containing chloramphenicol at 30 µg/ml.E. coli DH5α
Blue colonies appeared when transformed with pUC19 and plated on L-agar containing 100 µg/ml ampicillin and 40 µg/ml X-Gal.
Additional Product Information
Please refer to the product’s Certificate of Analysis for storage conditions, product components, and technical specifications.
Kit Components List and Certificates of Analysis are available under the Documents tab.
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