Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience

Thermo Fisher Scientific CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 660
• Functional Grade
• PerCP-eFluor 710
• Custom conjugation available

Product Specific Information

The monoclonal antibody DECMA-1 recognizes mouse, human, and canine CD324 (E-Cadherin, also known as epithelial cadherin or uvomorulin). E-Cadherin is a transmembrane glycoprotein involved in intercellular adhesion, sharing structural features with other cadherin family members (P and N cadherin). Its extracellular domain contains repeating Ca²⁺-binding motifs, while the cytoplasmic domain interacts with catenins (α, β, γ) and actinins, linking E-Cadherin to the cytoskeleton.

E-Cadherin is expressed in most epidermal cells, including melanocytes and keratinocytes, and localized at epithelial cell boundaries. It plays a critical role in maintaining tissue integrity. Loss of E-Cadherin function is associated with cancer progression.

Note: E-Cadherin is sensitive to trypsin; avoid trypsin exposure.

The DECMA-1 antibody demonstrates functional activity by disrupting adhesion in human, mouse, and dog cells.

Applications Reported: Flow cytometry, Western blotting, and immunohistochemistry (frozen tissue).

Applications Tested: Flow cytometric analysis of MDCK cells. Optimal staining achieved by intracellular staining due to variable surface expression. Recommended ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration is advised for optimal assay performance.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the calcium-dependent adhesion protein superfamily. It is a cell-cell adhesion glycoprotein with five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. Mutations are linked to gastric, breast, colorectal, thyroid, and ovarian cancers. Loss of E-Cadherin function promotes tumor invasion and metastasis. The ectodomain mediates bacterial adhesion, while the cytoplasmic domain enables internalization. The human CDH1 gene on chromosome 16 encodes E-Cadherin.

E-Cadherin is essential for tissue architecture and integrity maintenance. Reduced expression correlates with increased tumor invasiveness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.