Thermo Fisher Scientific CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (CFTR, 1643)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 1–2 µg/10^6 cells

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 min.

Target Information

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations in the CFTR gene, which encodes a cAMP-regulated chloride channel.
Approximately 70% of CF cases share a deletion of phenylalanine at position 508 (ΔF508), leading to abnormal chloride transport.
This mutation is often lethal due to lung and liver disease. Interestingly, CFTR also serves as an entry point for Salmonella typhi into intestinal epithelial cells, and ΔF508 heterozygote and homozygote mice show 86% and 100% reductions in S. typhi intestinal submucosal uptake.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.