Thermo Fisher Scientific CD3e Monoclonal Antibody (UCHT1)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Flow Cytometry (Flow)

1:50-1:100

View 15 publications 15 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Chimpanzee, Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD3,uniProtId:P07766-1,ncbiNodeId:9606,antigenRange:1-207,antigenLength:207,antigenImageFileName:MA1-80537_CD3_P07766-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80537_CD3_P07766-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:CD3,uniProtId:P04234-1,ncbiNodeId:9606,antigenRange:1-171,antigenLength:171,antigenImageFileName:MA1-80537_CD3_P04234-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80537_CD3_P04234-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:CD3,uniProtId:P09693-1,ncbiNodeId:9606,antigenRange:1-182,antigenLength:182,antigenImageFileName:MA1-80537_CD3_P09693-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80537_CD3_P09693-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:CD3,uniProtId:P20963-1,ncbiNodeId:9606,antigenRange:1-164,antigenLength:164,antigenImageFileName:MA1-80537_CD3_P20963-1_House_mouse.svg,antigenImageFileNamePDP:MA1-80537_CD3_P20963-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_928100

Product Specific Information

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD3 antibody, clone UCHT1 recognizes the human T-cell surface glycoprotein CD3 epsilon chain, also known as T-cell surface antigen T3/Leu-4 epsilon chain or CD3ε.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.