Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (KT15)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Mouse

Published species

Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KT15

Immunogen

T cell clone, C6 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01731-1,ncbiNodeId:10090,antigenRange:1-247,antigenLength:247,antigenImageFileName:MA1-80557_CD8_P01731-1_Rat.svg,antigenImageFileNamePDP:MA1-80557_CD8_P01731-1_Rat_PDP.jpeg,sortOrder:2},{targetFamily:CD8,uniProtId:P10300-1,ncbiNodeId:10090,antigenRange:1-213,antigenLength:213,antigenImageFileName:MA1-80557_CD8_P10300-1_Rat.svg,antigenImageFileNamePDP:MA1-80557_CD8_P10300-1_Rat_PDP.jpeg,sortOrder:5}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929627

Product Specific Information

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti mouse CD8 alpha, clone KT15, recognizes the alpha chain of mouse CD8.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.