Thermo Fisher Scientific ETFA Polyclonal Antibody
ETFA 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ELISA, IP 등에 사용 가능. 인간, 마우스, 랫트 반응성. PBS/glycerol buffer에 보관되며 -20°C에서 안정. 미토콘드리아 매트릭스 단백질 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–333 of human ETFA (NP_000117.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914695 |
Product Specific Information
Positive test controls include:
MCF7, A-549, SW620, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver.
The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence:
MFRAAAPGQL RRAASLLRFQ STLVIAEHAN DSLAPITLNT ITAATRLGGE VSCLVAGTKC DKVAQDLCKV AGIAKVLVAQ HDVYKGLLPE ELTPLILATQ KQFNYTHICA GASAFGKNLL PRVAAKLEVA PISDIIAIKS PDTFVRTIYA GNALCTVKCD EKVKVFSVRG TSFDAAATSG GSASSEKASS TSPVEISEWL DQKLTKSDRP ELTGAKVVVS GGRGLKSGEN FKLLYDLADQ LHAAVGASRA AVDAGFVPND MQVGQTGKIV APELYIAVGI SGAIQHLAGM KDSKTIVAIN KDPEAPIFQV ADYGIVADLF KVVPEMTEIL KKK
Target Information
ETFA participates in catalyzing the initial step of mitochondrial fatty acid beta-oxidation.
It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase.
Defects in electron-transfer-flavoprotein are implicated in type II glutaricaciduria, where multiple acyl-CoA dehydrogenase deficiencies lead to excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
Two transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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