Thermo Fisher Scientific GluD1 Polyclonal Antibody
Thermo Fisher Scientific의 GluD1 Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 고품질 항체로 WB, IHC, ICC, ELISA, IP 등 다양한 분석에 적합합니다. 재조합 단백질 면역원 기반으로 제작되어 높은 특이성과 재현성을 제공합니다.
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Thermo Fisher Scientific GluD1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 54–242 of human GLUD1 (NP_005262.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.02 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914693 |
Product Specific Information
Positive test controls include: HeLa, HepG2, NIH/3T3, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain, Rat kidney.
The target is usually found in the following locations: Mitochondrion matrix.
Immunogen sequence:
SEAVADREDD PNFFKMVEGF FDRGASIVED KLVEDLRTRE SEEQKRNRVR GILRIIKPCN HVLSLSFPIR RDDGSWEVIE GYRAQHSQHR TPCKGGIRYS TDVSVDEVKA LASLMTYKCA VVDVPFGGAK AGVKINPKNY TDNELEKITR RFTMELAKKG FIGPGIDVPA PDMSTGEREM SWIADTYAS
Target Information
Glutamate dehydrogenase (GluD1) is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.
This enzyme plays an important role in regulating amino acid-induced insulin secretion and is allosterically activated by ADP while inhibited by GTP and ATP.
Activating mutations in this gene are a common cause of congenital hyperinsulinism.
Alternative splicing results in multiple transcript variants.
The related glutamate dehydrogenase 2 gene on the human X-chromosome originated via retrotransposition and encodes a soluble form of glutamate dehydrogenase.
Related pseudogenes have been identified on chromosomes 10, 18, and X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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