Thermo Fisher Scientific GluD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 54-242 of human GLUD1 (NP_005262.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GluD1,uniProtId:P00367-1,ncbiNodeId:9606,antigenRange:54-242,antigenLength:558,antigenImageFileName:PA5-121121_GluD1_P00367-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121121_GluD1_P00367-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914693

Product Specific Information

Positive test controls include: HeLa, HepG2, NIH/3T3, U-251MG, Mouse liver, Mouse brain, Rat liver, Rat brain, Rat kidney. The target is usually found in the following locations: Mitochondrion matrix.

Immunogen sequence: SEAVADREDD PNFFKMVEGF FDRGASIVED KLVEDLRTRE SEEQKRNRVR GILRIIKPCN HVLSLSFPIR RDDGSWEVIE GYRAQHSQHR TPCKGGIRYS TDVSVDEVKA LASLMTYKCA VVDVPFGGAK AGVKINPKNY TDNELEKITR RFTMELAKKG FIGPGIDVPA PDMSTGEREM SWIADTYAS

Target Information

Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.