Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), PE, eBioscience
Human 및 Mouse c-Maf 단백질을 인식하는 sym0F1 클론 단일클론 항체. PE 형광체 결합으로 유세포분석(Flow Cytometry)에 최적화. IL-4, IL-21 등 Th2, Th17, Tfh 관련 전사인자 연구에 적합. 4°C 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): 5 µL (0.06 µg)/test
- View 11 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572746 |
Additional Formats
- Alexa Fluor 488
- Brilliant Violet 421
- Brilliant Violet 480
- eFluor 450
- eFluor 660
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays a role in Th17 and Tfh cell differentiation and function by driving IL-21 expression and promoting IL-23R and CXCR5 expression.
Sym0F1 does not cross-react with MafA or MafB.
Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometry of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00) and protocol.
Recommended usage: 5 µL (0.06 µg)/test in a final volume of 100 µL.
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
c-Maf is the cellular counterpart of oncogenic v-maf, belonging to the basic region leucine zipper transcription factor family. It interacts with LRPICD and exists in two forms (long and short). c-Maf regulates IL-4 in Th2 cells, crystalline genes in lens fiber cells, insulin gene in islets, and p53/L7 through Maf recognition element (MARE). It plays a key role in Th2 differentiation, hematopoiesis, and IL-10 gene expression in LPS-activated macrophages. Chromosomal aberrations involving maf occur in multiple myeloma, and expression is observed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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