Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE, eBioscience
인간 및 생쥐 IRF4 단백질을 인식하는 PE 결합 단클론 항체입니다. 세포내 염색 및 유세포분석(Flow Cytometry)에 적합합니다. 0.125 µg/test로 사용 권장되며, 4°C 암소 보관이 필요합니다. 면역세포 기능 연구 및 혈액암 관련 연구에 활용됩니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IRF4 Monoclonal Antibody (3E4), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 22 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E4 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853179 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- Brilliant Violet 480
- Brilliant Violet 650
- eFluor 450
- eFluor 660
- FITC
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4), a 52 kDa transcription factor involved in B cell, T cell, and macrophage function.
- In B cells, IRF4 is highly expressed in mature plasma cells and regulates genes such as Prdm1 (Blimp1).
- In T cells, it contributes to Treg, Th2, Th9, and Th17 cell differentiation and function, and induces Blimp1 and IL-10 expression.
- In macrophages, IRF4 is involved in polarization and immune regulation.
- IRF4 expression is also associated with several blood-related cancers.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on stimulated normal human peripheral blood cells using Foxp3/Transcription Factor Buffer Set (Product #00-5523-00) following BestProtocols® for intracellular (nuclear) proteins.
- Recommended use: ≤ 0.125 µg/test (in 100 µL final volume).
- Cell number: 10⁵–10⁸ cells/test (empirically determined).
- Antibody titration recommended for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
The IRF (interferon regulatory factor) family contains transcription factors with a tryptophan pentad repeat DNA-binding domain.
- Regulates interferon responses and interferon-inducible genes.
- IRF4 is lymphocyte-specific and negatively regulates TLR signaling, modulating innate and adaptive immunity.
- Chromosomal translocation t(6;14)(p25;q32) involving IRF4 and IgH may cause multiple myeloma.
- Alternative splicing variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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