Thermo Fisher Scientific CLCNKA Polyclonal Antibody
Rabbit polyclonal antibody targeting human CLCNKA, validated for WB, IHC(P), and ICC/IF. Detects endogenous CLCNKA protein. Supplied as liquid, 1 mg/mL, affinity purified, in PBS with 50% glycerol. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CLCNKA (Accession P51800), corresponding to amino acid residues E583–L633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850952 |
Product Specific Information
Antibody detects endogenous levels of total CLCNKA.
Target Information
CLC-KA is a kidney-specific chloride channel that mediates transepithelial chloride transport in the thin ascending limb of the Henle loop in the inner medulla. It plays a crucial role in urine concentration.
The gene encoding human CLC-KA maps to chromosome 1p36. Mutations in this gene may be associated with nephrogenic diabetes insipidus in cases lacking mutations in the vasopressin V2 receptor and AQP2 water channel.
CLC-KB mediates basolateral chloride ion efflux in the thick ascending limb and distal nephron segments. Mutations in the CLC-KB gene cause type III Bartter’s syndrome, characterized by renal salt-wasting and low blood pressure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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