Takara SMART-Seq Total RNA High Input (RiboGone Mammalian)

NOTE: SMART-Seq Total RNA High Input (RiboGone Mammalian) is an equivalent replacement for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

1. For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
2. New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)

SMART-Seq Total RNA High Input (RiboGone Mammalian) provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation with the Unique Dual Index Kits (Cat. # 634752–634756, sold separately) in around 5 hours.

NOTE: SMART-Seq Total RNA High Input (RiboGone Mammalian) is an equivalent replacement for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

1. For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
2. New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)

SMART-Seq Total RNA High Input (RiboGone Mammalian) provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation.

Because rRNA can comprise ~90% or more of total RNA, effective depletion of rRNA prior to library generation can lower sequencing costs and improve mapping statistics. SMART cDNA synthesis technology followed by PCR complete a streamlined process that performs strand information and eliminates downstream library prep by incorporating indexes and adapters from our Unique Dual Index Kits (Cat. # 634752–634756, sold separately) during the reverse transcription and amplification steps.

The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.

Alternate kits for other input amounts:

• For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
• For 10–100 ng of total RNA, we recommend the SMART-Seq Total RNA Mid Input

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Overview

• Strand information—Identify each transcript’s strand of origin with >99% accuracy.
• Fast, streamlined protocol—Go from start to finish in ~5 hours.
• Broad input range—Use 100 ng–1 µg of total RNA from human, mouse, or rat.
• Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
• Seamless integration with Illumina sequencing—Generate up to 384 uniquely indexed, Illumina-ready libraries.

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

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Applications

• RNA-seq for mammalian samples on Illumina platforms
• NGS library generation that retains strand information
• Analysis of coding and noncoding sequence information

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.