Takara Tet-On 3G tetracycline-inducible expression systems

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Tet-On 3G tetracycline-inducible gene expression systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced systems with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

Learn more about the high sensitivity of the transactivator, and the low background from the tight promoter in the Tet system learning center.

Choice of Tet-On 3G vector formats

There is a range of available Tet-On 3G vector formats:

• Tet-One systems (listed elsewhere) provide a complete inducible expression system on a single vector.
• The Tet-On 3G Inducible Expression System (EF1alpha Version), our EF1-alpha promoter version of the Tet-On 3G system, provides consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
• Bidirectional promoter versions of the system allow you to induce expression of two genes from the same promoter—either two transgenes of your choosing, or a single transgene and a fluorescent protein. If your cells turn red or green after adding Dox, this confirms that your gene has been turned on and that you have selected a high-performing inducible clone.
• Using IRES technology, we`ve married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry), and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1).
• Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.

Use tetracycline-approved serum for optimal results

With the greatly increased sensitivity of the Tet-On 3G system, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Takara Bio performs actual inducibility tests on a sensitive Tet-inducible cell line in order to provide an absolute guarantee that our serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G system is supplied with 50 ml of our premium Tet System Approved FBS.

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