Takara Yeast minimal media/synthetic-defined (SD) premixes

Yeast minimal media and synthetic defined premixes (SD) come in highly convenient single-use pouches. Simply empty the content of the pouch into your media flask/bottle, add 0.5 L of dH2O, and sterilize. No weighing of individual supplements, combining, or pH adjustment is required.

Yeast minimal media and synthetic defined premixes (SD) come in highly convenient single-use pouches. Simply empty the content of the pouch into your media flask/bottle, add 0.5 L of dH2O, and sterilize. No weighing of individual supplements, combining, or pH adjustment is required.

With or without agar

All minimal yeast media pouch mixes are available either with agar or without (broth).

Minimal media

Synthetic defined (SD) broths and agar minimal media consist of a carbon source (glucose), a nitrogen source, and amino acids. In each particular SD mix, certain amino acids have been “dropped out”. For example, SD/-Leu/-Trp contains all essential amino acids except for leucine and tryptophan.

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Overview

• Minimal media for yeast—conveniently premixed
• Large selection of dropout mixes
• No weighing, mixing, or pH adjustment required
• Simply mix each pouch with 500 ml water and sterilize
• Proven track record—15 years of yeast media sales

More Information

Applications

• Culturing auxotrophic yeast strains
• Yeast transformation and selection
• Yeast two-hybrid screening
• Yeast one-hybrid screening

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.