Thermo Fisher Scientific Mesothelin Monoclonal Antibody (K1)

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:40

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

K1

Immunogen

Spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumor cell line derived from poorly differentiated adenocarcinoma of the ovary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.3 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_794846

Product Specific Information

Recommended positive controls: Tonsil, lymph node and OVCAR-3 cells (human).

Target Information

Originally identified as a differentiation antigen of mesotheliomas, ovarian cystadenocarcinomas, and pancreatic adenocarcinomas, Mesothelin is a glycosylphosphatidylinositol (GPI)-anchored, cell-surface glycoprotein predominantly secreted by cells of the mesothelium. Although Mesothelin is expressed at restricted levels by normal mesothelial cells of the pleural,pericardial, and peritoneal membranes, aberrant expression has been documented in the aforementioned cancers, as well as in endometriod uterine adenocarcinomas and squamous cell carcinomas of the esophagus, stomach, lung, and cervix. Proteolytic cleavage of Mesothelin yields a soluble, polypeptide fragment designated megakaryocyte potentiating factor (MPF) based on its ability to stimulate megakaryocyte colony-forming activity of murine interleukin-3 in murine bone marrow cell cultures. Originally isolated from the HPC-Y5 pancreatic cell line, MPF has been suggested to play a role in the proliferation and differentiation of megakaryoctyes, and the regulation of resultant platelet production. While the biological functions of both Mesothelin and MPF remain speculative, high-levels of expression in cancerous tissues compared to limited distribution in normal tissues strongly suggests their involvement in tumorigenesis. Both have been demonstrated to promote tumor cell proliferation, migration,anchorage-independent growth, and tumor progression; demonstrating their involvement in heterotypic cell adhesion and the metastatic spread of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.