Thermo Fisher Scientific P-Glycoprotein Monoclonal Antibody (JSB-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:40

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JSB-1

Immunogen

Multidrug-resistant Chinese hamster ovary cell line (CHrC5).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

67 µg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_795163

Product Specific Information

This antibody is specific for the MDR1 isoform of P Glycoprotein. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positives taining for P glycoprotein.

For optimal Western blot staining, the primary antibody should be incubated 20-60 minutes at room temperature. Sections should be pretreated with an antigen unmasking solution.

Target Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.