Thermo Fisher Scientific Tubulin, beta Monoclonal Antibody (TUBB/4470)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• 1–2 µg/mL

Product Specifications

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications remains unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, and this phosphorylation inhibits tubulin incorporation into microtubules.

Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin dimers (alpha and beta subunits, each ~55 kDa). They play essential roles in chromosome segregation during mitosis, intracellular transport, ciliary and flagellar movement, and maintaining cytoskeletal structure. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.