Thermo Fisher Scientific PILRB Polyclonal Antibody
Thermo Fisher Scientific의 PILRB Polyclonal Antibody는 인간 PILR-beta 단백질을 검출하는 항체로, Western blot 및 유세포분석에 적합합니다. 고순도의 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공되어 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Flow Cytometry (Flow) | 2.5 µg per million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human PILR-beta isoform 1 (Gln20–Ala189) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576625 |
Product Specific Information
This antibody detects recombinant human PILR-beta in direct ELISAs and Western blots.
In Western blots, approximately 10% cross-reactivity with recombinant human PILR-alpha is observed and less than 5% cross-reactivity with recombinant mouse (rm) PILR-beta, rmPILR-alpha, and rmPILR-L is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Paired receptors consist of highly related activating and inhibitory receptors involved in immune regulation. PILRB functions as an activating receptor associating with ITAM-bearing adapter molecules on the cell surface.
Cell signaling pathways depend on the balance between activating and inhibiting processes. SHP-1-mediated dephosphorylation of tyrosine residues plays a central regulatory role.
PILRalpha mediates inhibition, while PILRbeta mediates activation. These paired receptor genes are tandemly arranged on chromosome 7.
This gene encodes the non-ITIM-bearing activating receptor variant with a truncated cytoplasmic tail. Alternative splicing yields multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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