Thermo Fisher Scientific C. botulinum BoNT-A Light Chain Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant Clostridium BoNT-A Light Chain Met1-Phe425

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610698

Product Specific Information

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant C. botulinum BoNTA Heavy Chain is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

Botulinum neurotoxin type A is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum. BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain. BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction. E. coli-expressed recombinant light chains are active proteases. However, they are not toxic because they cannot enter into host cells in the absence of the heavy chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.