Thermo Fisher Scientific C. botulinum BoNT-A Light Chain Polyclonal Antibody
C. botulinum BoNT-A Light Chain을 인식하는 sheep polyclonal antibody로 Western blot 및 Immunoprecipitation에 적합. E. coli 유래 recombinant immunogen 사용. 높은 특이성과 낮은 교차반응. 연구용으로만 사용 가능.
- 카탈로그번호
- PA548053
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific C. botulinum BoNT-A Light Chain Polyclonal Antibody
Applications
Western Blot (WB)
- Tested Dilution: 0.1 µg/mL
Immunoprecipitation (IP)
- Tested Dilution: 25 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant Clostridium BoNT-A Light Chain (Met1–Phe425) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610698 |
Product Specific Information
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant C. botulinum BoNT-A Heavy Chain is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Botulinum neurotoxin type A (BoNT-A) is one of seven serotypes of Botulinum Neurotoxins produced by various strains of Clostridium botulinum.
BoNTs are synthesized as inactive single-chain precursors and activated by proteolytic cleavage into disulfide-linked two-chain proteins.
- Light Chain (50 kDa): Catalytic domain
- Heavy Chain (100 kDa): Translocation and receptor binding domains
BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins, blocking acetylcholine release at the neuromuscular junction and causing flaccid paralysis.
E. coli-expressed recombinant light chains are active proteases but non-toxic due to lack of cell entry capability without heavy chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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