Thermo Fisher Scientific c-Fos Monoclonal Antibody (2H2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2H2

Immunogen

Recombinant full length human c-FOS expressed in and purified from E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~1 mg/mL

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942669

Product Specific Information

Specific for endogenous levels of the ~50 kDa c-FOS protein.

Target Information

FBJ murine osteosarcoma viral oncogene homolog is commonly known as Cellular protooncogene Fos (c-Fos). It belongs to the fos transcription factor family and is encoded by the C-FOS gene located on chromosome 14. c-Fos heterodimerizes with c-Jun to form the Activator Protein -1 (AP-1) transcription factor which regulates transcription of several genes that play an important role in cellular signal transduction, cell proliferation and differentiation. c-Fos has been shown to be phosphorylated by various stimuli including growth factors and insulin at atleast seven different sites. The best known upstream kinase for phosphorylation at Threonine 232 is ERK MAPK which regulates localization of c-Fos to the nucleus and is thus important for c-Fos induced transcriptional activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.