Thermo Fisher Scientific LDLR Monoclonal Antibody (472413)
인간 LDL 수용체(LDLR)를 인식하는 마우스 단클론 항체로, Western blot, ELISA, Flow cytometry, Immunoprecipitation에 적합합니다. 비보존제 제형으로 동결건조 상태 제공되며, 재구성 후 0.5 mg/mL 농도로 사용 가능합니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Flow Cytometry (Flow) | 0.25 µg per million cells |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 2–8 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 472413 |
| Immunogen | CHO-derived recombinant human LDL R (Ala22–Arg788) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609816 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LDL R, recombinant human LRP-5, or recombinant mouse LRP-6 is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands.
LDL binds to the receptor at the cell membrane and is internalized into lysosomes, where the protein is degraded and cholesterol becomes available for regulation of HMG CoA reductase, the rate-limiting enzyme in cholesterol synthesis.
Mutations in the LDL-R gene cause familial hypercholesterolemia, an autosomal dominant disorder.
LDL-R also serves as an entry factor for the Hepatitis C virus, along with SCARB1, CLDN1, and CD81.
At least three isoforms of LDL-R are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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