Thermo Fisher Scientific LDLR Monoclonal Antibody (472413)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

472413

Immunogen

CHO-derived recombinant human LDL R Ala22-Arg788 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LDLR,uniProtId:P01130-1,ncbiNodeId:9606,antigenRange:22-788,antigenLength:860,antigenImageFileName:MA5-23916_LDLR_P01130-1_House_mouse.svg,antigenImageFileNamePDP:MA5-23916_LDLR_P01130-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609816

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.