Thermo Fisher Scientific mCherry Chimeric Recombinant Mouse Monoclonal Antibody (16D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Flow Cytometry (Flow)

0.5 µg/test

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

16D7

Immunogen

Full-length protein mCherry

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

Product Specific Information

This antibody has Rat Variable heavy and light chains, and Mouse constant domains. It recognizes mCherry protein. This chimeric antibody is recognized by anti-mouse secondary antibodies.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.