Thermo Fisher Scientific NEK8 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 270-390 of human NEK8 (NP_8354641) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEK8,uniProtId:Q86SG6-1,ncbiNodeId:9606,antigenRange:270-390,antigenLength:692,antigenImageFileName:PA5-116501_NEK8_Q86SG6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116501_NEK8_Q86SG6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901132

Product Specific Information

Positive Samples: HeLa, Jurkat, K-562, Mouse liver

Immunogen sequence: GSVRMRRAEK SVAPSNTGSR TTSVRCRGIP RGPVRPAIPP PLSSVYAWGG GLGTPLRLPM LNTEVVQVAA GRTQKAGVTR SGRLILWEAP PLGAGGGSLL PGAVEQPQPQ FISRFLEGQS G

Target Information

NEK8 serine/threonine kinase or NIMA-related kinase 8 is related to NIMA (never in mitosis, gene A) of Aspergillus nodulins and may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development. NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins. Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia.Defects in NEK8 are the cause of nephronophthisis type 9 (NPHP9) [MIM:613824]. NPHP9 is an autosomal recessive disorder resulting in end-stage renal disease. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.