Thermo Fisher Scientific NEK8 Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal NEK8 항체로 WB, ICC/IF, ELISA에 사용 가능. Recombinant NEK8(aa 270-390)을 면역원으로 제작. 액상 형태, Affinity chromatography로 정제, PBS/glycerol buffer에 보관. 세포주 및 조직에서 NEK8 단백질 검출에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 270–390 of human NEK8 (NP_835464.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901132 |
Product Specific Information
- Positive Samples: HeLa, Jurkat, K-562, Mouse liver
- Immunogen sequence:
GSVRMRRAEK SVAPSNTGSR TTSVRCRGIP RGPVRPAIPP PLSSVYAWGG GLGTPLRLPM LNTEVVQVAA GRTQKAGVTR SGRLILWEAP PLGAGGGSLL PGAVEQPQPQ FISRFLEGQS G
Target Information
NEK8 (NIMA-related kinase 8) is a serine/threonine kinase related to NIMA of Aspergillus. It may play a role in cell cycle progression from G2 to M phase and is part of a complex of ciliary proteins required for renal and cardiovascular development. NEK8 interacts with polycystin signal transduction pathways and may regulate ciliary biogenesis through targeting of proteins to the cilia.
Defects in NEK8 cause nephronophthisis type 9 (NPHP9, MIM:613824), an autosomal recessive disorder leading to end-stage renal disease characterized by tubular basement membrane thickening, interstitial fibrosis, and medullary cysts.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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