Thermo Fisher Scientific NeuN Polyclonal Antibody
Rabbit polyclonal antibody recognizing NeuN (Fox-3/RBFOX3) for detection in human, mouse, and rat samples. Validated for WB, IHC, ICC/IF, and ELISA. Supplied as liquid, affinity-purified, and stored in PBS with 50% glycerol for reliable neuronal marker...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–220 of NeuN (NP_0010760441) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.72 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901131 |
Product Specific Information
- Positive Samples: Mouse brain, Rat brain
- Immunogen sequence:
MAQPYPPAQY PPPPQNGIPA EYAPPPPHPT QDYSGQTPVP TEHGMTLYTP AQTHPEQPGS EASTQPIAGT QTVPQTDEAA QTDSQPLHPS DPTEKQQPKR LHVSNIPFRF RDPDLRQMFG QFGKILDVEI IFNERGSKGF GFVTFETSSD ADRAREKLNG TIVEGRKIEV NNATARVMTN KKTGNPYTNG WKLNPVVGAV YGPEFYAVTG FPYPTTGTAV
Target Information
Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein containing one RRM (RNA recognition motif) domain. It localizes to both the nucleus and cytoplasm and is suggested to regulate alternative splicing events.
Fox-3 is encoded by a gene located on human chromosome 17, which contains over 2.5% of the human genome and encodes more than 1,200 genes. Two key tumor suppressor genes associated with chromosome 17 are p53 and BRCA1.
- p53 maintains genomic integrity by regulating DNA repair and apoptosis.
- BRCA1 is involved in DNA repair and is linked to early-onset breast and ovarian cancers, as well as other malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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